Fig. 4. Pharmacological inhibition of Notch signalling impairs the ability of ß-catenin to induce new hair follicles. (A) Lysates from total skin of wild-type mice treated with 1 mg DAPT or vehicle alone were analysed by western blotting with antibodies to activated Notch (NICD) and ß-tubulin. (B,C) K14N ß-cateninER mouse keratinocytes were cultured with or without 4OHT, DAPT or 4OHT + DAPT for 72 hours, then co-transfected with (B) Renilla luciferase (pRL) and Hes1 luciferase (Hes1-Luc), with or without full-length Notch1 (hNotch1) or (C) with pRL and TOPFLASH or FOPFLASH luciferase reporters. Data are expressed as average light units ±s.d., relative to the Renilla luciferase control. ^*P<0.011. n, number of replicate samples. (D-G) H&E (D,E) and anti-CDP (F,G) stained sections of back skin from K14N ß-cateninER mice treated with 4OHT (D,F) or 4OHT + DAPT (E,G). Arrows indicate ectopic hair follicles. Scale bars: 100 µm.