Fig. 5. Overexpression of SCF results in abnormal morphology of the male X chromosome but not of autosomes or the female X chromosomes. (A) Polytene chromosomes prepared from an yw control male were labeled with DAPI. (B) Chromosomes prepared from a female of the transgenic SCF-overexpression line were labeled with DAPI. (C-F) Chromosomes prepared from males of the transgenic SCF-overexpression line were labeled with DAPI (C,E; green in F) and anti-MSL-1 (D) or anti-SCF (magenta in F) antibody. (G,H) The male X chromosome appears normal in the line overexpressing both SCF and ISWI (G), and upon overexpresssion of SCF in the mof¹ mutant background (H). (I) Acetylation of H4K16 was not detectable on polytene chromosomes in mof¹. The chromosomes shown in I were labeled with anti-acetylated H4K16 antibody. Arrows mark the X chromosome.