Fig. 4. Effect of trap100 antisense morpholino oligonucleotide injection on jaw, heart and enteric neuron development. (A,B) Lateral views of the heads of 96 hpf trap100 morphant (A) and lsn mutant (B) embryos. Arrowheads indicate the recessed jaw; arrows indicate cardiac oedema. (C,D) Lateral views of the intestines of 96 hpf embryos stained with anti-Hu antibody. Arrowheads indicate the end of the intestine. The lack of melanophores in morphant the embryo (A) is not due the effect of the trap100 morpholino. Embryos injected with the trap100 morpholino were obtained from an incross of nacre homozygous fish (Lister et al., 1999) and lack melanophores because of a mutation in mitfa.