Fig. 11. Analysis of DIF induction of ecmA expression in a control and a dimB- mutant. A dimB-null and a control (random integrant) strain were plated at 10⁵ cells/cm², in the presence of 50 µM cerulenin, and treated with the indicated amounts of DIF-1. Total cellular RNA was extracted and the relative abundances of the ecmA and Ig7 RNAs (a constitutively expressed gene, used as a loading control) in the two strains determined by semi-quantitative RT-PCR.