Fig. 2. Differential accumulation of Hh-GFP versus HhN-GFP and Hhc85s-GFP in the plasma membrane of shi^ts1 mutant clones. (A-E) shi^ts1mutant clones induced in UAS-Hh-GFP/hhGal4 (A,D), UAS-HhN-GFP/hhGal4 (B,E) and Hhc85s-GFP/hhGal4 (C) wing imaginal discs. Hh-GFP accretions were only observed in the basolateral plasma membrane of the A compartment cells (arrowheads in A), while HhN-GFP (B) and Hhc85s-GFP (C) were detected only in the apical part of the A compartment cells. (D,E) Transverse sections of the shi^ts1mutant clones in A,B, respectively. Arrows indicate the apical parts and arrowheads indicate the basolateral parts of the cells. There is basolateral accumulation of Hh (D) and apical accumulation of HhN (E). The AP boundary is indicated with a broken line.