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Figure 6


Fig. 6. Oligodendrocytes are absent from Olig1-DTA embryos but astrocytes are generated normally. Antibodies against Pdgfr{alpha} (A-F), Olig2 (G-L), MBP (M-P) and Gfap (Q-T) were used for immunostaining sections from the thoracic region of the spinal cord. OPCs expressing Pdgfr{alpha} (A-C), and mature oligodendrocytes expressing MBP (M,N, arrowheads), are missing in Olig1-DTA embryos (D-F,O,P). Olig2-expressing cells, normally scattered in gray and white matter (G-I), are greatly reduced at E14.0 to E18.0 (J; K,L, insets, arrowheads) in Olig1-DTA embryos. Astrocytes expressing Gfap are visible both in control (arrowheads in Q,R) and in Olig1-DTA (arrowheads in S,T) animals. Scale bars: in J, 100 µm for A,D,G,J; in T, 200 µm for B,C,E,F,H,I,K-T.