Fig. 6. Skeletal muscle gene expression of Mck, Mrf4, Myod1 and Myf5 in Myog^flox/flox and Myog^flox/flox mice. Quantitative RT-PCR was performed on RNA prepared from hindlimb muscle at 3 days (A) and 2 weeks (B) of age. In each case, the ratio of myogenin to ribosomal protein L7 RNA for one RNA sample with a Myog^flox/flox genotype was arbitrarily set to 1, and all other ratios from both Myog^flox/flox and Myog^flox/flox genotypes were normalized to that value. The median value for each genotype is shown as a black dot. Asterisks indicate significant differences between the two genotypes. Error bars indicate one s.d. from the median.