Fig. 2. aak-2 positively regulates lifespan and dauer maintenance. (A) daf-2(e1370) (n=100), daf-2(e1370); aak-2(rr48) (n=98) and daf-2(e1370); aak-2(ok524) (n=60) individual larvae were assayed for dauer lifespan (days). (B) Whereas most daf-7(e1372) (n=500) dauer larvae remain at this stage at 25°C, the majority of daf-7(e1372); aak-2(rr48) (n=500), or daf-7(e1372); aak-2(ok524) (n=400) dauer larvae recover prematurely within 5 days. (C) aak-2 is required for the full adult lifespan extension induced by compromised insulin-like signalling. The graph marks the time (day) of the temperature up-shift (corresponding to the L4 larval stage). Each line represents the average of three independent replicate experiments (each with n=50), not performed in parallel, except for daf-2(e1370); aak-2(ok524), for which we did one single experiment (n=50). Error bars represent standard deviation between cohorts of (A) 10 or (B) 100 individuals.