Fig. 1. XHas2 is transcriptionally activated by activin. (A,A') Ectopic XHas2 mRNA expression in activin-injected embryos detected by whole-mount in situ hybridization. The site of injection is visualized by the Red-gal staining (red arrow). A, dorsal view; A', lateral view. (B) Comparison of the expression profile of XBrachyury and XHas2 in animal caps following activin treatment. XODC was used as positive control. RT-indicates representative samples of RNA from activin-treated animal caps, processed without reverse transcriptase and subsequently used as a negative control for XHas2, XBrachyury and XODC amplification.