Fig. 6. hydkk1/2/4 and hywnt3a expression in regenerating epithelial sf-1 polyps. (A) Quantification of residual hydkk1/2/4-positive gland cells in body column pieces (left); n=6, 3, 5, 48, 37 and 22 with increasing time. Efficiency of head regeneration was measured as the average number of tentacles per regenerate (right); n=20, 101, 69, 178 and 140. Bars indicate s.d. Animals were cut at 50% body length and regenerated at least 4 hours prior to in situ hybridization and up to 9 days for determination of regeneration behaviour. (B-G) hydkk1/2/4 expression in individual regenerates, 4 hours (B-D) and 9 days (E-G) after head removal. (H-J) Ectopic hywnt3a expression in regenerating epithelial sf-1 polyps (5-9 days regeneration).