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Fig. 1. Sequence and expression of Arabidopsis BRICK1. (A)
Alignment of maize and Arabidopsis BRICK1 protein sequences. Dark and
light shading highlight identical and similar residues, respectively. The
Arabidopsis BRK1 protein sequence is supported by the sequence of
full-length cDNA clone RAFL04-19-H22 (GenBank Accession Numbers AV821991 and
AV782595). Asterisks indicate the locations of premature stop codons in mutant
alleles brk1-1 and brk1-2. (B) RT-PCR analysis
illustrating expression of Arabidopsis BRK1 and ubiquitin in all
tissues analyzed (FB, flower buds; RT, root tips; ES, expanding siliques; EC,
expanding cotyledons; MC, mature cotyledons; EL, expanding leaves; ML, mature
leaves).
