Fig. 8. Loss of SCAR1 protein in scar1 and brk1 mutants. Western blots of anti-SCAR1 immunoprecipitates from shoot tips of 3-week-old seedlings probed with anti-SCAR1. Lane 1, wild type; lane 2, scar1 mutant; lane 3, brk1-1 mutant. Black arrowhead indicates SCAR1 protein detected in wild-type extract, which is undetectable in scar1 extracts and dramatically reduced in brk1-1 extracts (the smaller protein also recognized by this antibody serves as a loading control).