Fig. 7. MARCM analysis of Pc and E(z) in the mosaic fly brains. (A-G) Composite confocal images of various MB clones at the wandering larval (A,B) or adult (C-G) stage. A gigantic loose structure is present in the larval Pc mutant Nb clone (arrow in A) versus the dense well-defined calyx in the larval E(z) mutant Nb clone (arrow in B). Numerous ectopic neurites are present in both Pc (C) and E(z) (D) mutant adult Nb clones, and the dendritic tree is over-elaborated in the adult Pc single-cell clone (arrowhead in F; compare with arrowheads in E and G). A calyx-like structure in the adult E(z) mutant Nb clone is shown in the inset in D. (H) Various glia-like E(z) mutant brain cells became ectopically labeled by GAL4-OK107. Genotypes: (A,C,F) hs-FLP,UAS-mCD8GFP/+; Pc^XT109,FRT2A/tubP-GAL80,FRT2A;GAL4-OK107/+; (B,D,G,H) hs-FLP,UAS-mCD8GFP/+;E(z)⁷³¹,FRT2A/tubP-GAL80,FRT2A;GAL4-OK107/+; and (E) hs-FLP,UAS-mCD8GFP/+;FRT2A/tubP-GAL80,FRT2A;GAL4-OK107/+. Scale bar: 20 µm.