Fig. 1. Migration pattern and characteristics of olfactory cortex neurons. (A,B) The dorsal part of the telencephalon in E11.75 rat embryos is labeled by electroporation of GFP-expressing vector (arrow in B). After electroporation, the embryos were cultured for 48 hours in whole-embryo culture system. (C,C') During WEC, GFP-labeled cells migrate dorsoventrally in the telencephalon (arrows in C,C'), and stop at the pallium-subpallium boundary (PSB, open arrow in C'). Immunostaining with anti-Pax6 antibody indicates that Pax6 is not detected in the GFP-positive migrating cells (arrows in C,C'). Inset in C is at higher magnification in C'. (D-F'') Immunostaining with anti-type III ß-tubulin (Tuj1, D-D''), anti-calbindin (CB, E-E'') and anti-reelin (Rln, F-F'') antibodies of GFP-labeled telencephalon. GFP-positive migrating neurons (green cells in D-F) are positive for ß-tubulin (D-D''), CB (E-E'') and Rln (F-F''). Di, diencephalons; Tel, telencephalon. Scale bars: 500 µm in B; 100 µm in C'; 20 µm in F.