Fig. 3. Actin cytoskeleton organization and -tubulin staining and Tau-GFP localization in wild-type and spn-F ovaries. (A,C,E,G,I) Wild-type egg chambers; (B,D,F,H,J) spn-F egg chambers. (A,B) Young egg chambers. -tubulin, red; DNA, blue. (C,D) Stage 9 egg chambers. -tubulin, red. Abnormal aggregates of tubulin are seen at the nuclear periphery in the mutant egg chambers (arrow in D). (E,F) Abnormal accumulation of Tau-GFP is visible, associated with the mutant oocyte nucleus. Tau-GFP, green; DNA, blue; nuclear membrane, red. (G,H) Tau-GFP forms a gradient from anterior to posterior at stage 9 in both wild type and mutant; however, an abnormal accumulation of Tau-GFP occurs at the oocyte nuclear membrane in the mutant. Tau-GFP, green; DNA, blue. (I,J) Abnormal clumps of actin can be seen in the mutant egg chamber (J), compared with wild type (I). Rhodamine-phalloidin staining, red; DNA, blue.