Fig. 3. The 6ß1 integrin is not expressed on the surface of Myf5-null MPCs. (A-D) Co-immunohistochemistry for ß1 (A,B) or 6 (C,D) and ß-gal (A-D) on transverse cryosections of E9.5 embryos. Inserts show a higher magnification of each epaxial lip. (A,C) Myf5^nlacZ/+ embryos express both ß1 (A) and 6 (C) on the surface of dermomyotomal (arrowheads) and ß-gal expressing myotomal cells (arrows). (B,D) Myf5^nlacZ/nlacZ embryos express both ß1 (B) and 6 (D) on dermomyotomal cells (arrowheads), but most delaminated ß-gal positive MPCs are negative (arrows). ep, epaxial; nt, neural tube; scl, sclerotome. Scale bars: 100 µm.