Fig. 2. Ac and Sc promote the survival of the AWM mechanosensory lineages. (A-E) Loss of ac and sc results in a dramatic decrease in the number of AWM mechanosensory neurons. Double-staining of the AWM of (A) y w (wild type), (B) sc^10-1/Y, (C) sc^10-1/Y; sens^E2/+ and (D) sc^10-1/Y; src/+ pupae at 24 hours APF for Su(H) (green) and Elav (red) indicates that the loss of neurons and socket cells in a sc^10-1 background is quite sensitive to sens gene dosage. Quantification of the number of mechanosensory neurons in A-D is shown in E. Five wings were analyzed for each genotype. The number of neurons in sc^10-1 is less than 10% of wild type, and is significantly different from sc^10-1/Y; sens^E2/+ (^*P<0.0001) and sc^10-1/Y; src/+ (^**P<0.005). Error bars indicate s.e.m. (F-K') Overexpression of the anti-apoptotic protein P35 in the WM rescues the sc^10-1 stout SO phenotype. (F,H) Close-up views of the AWM from a sc^10-1/Y (F) and a sc^10-1/Y; C96-GAL4 UAS-P35/+ (H) fly; (G,G',I,I',J-K') wings of 36- to 42-hour APF pupae doubly stained with anti-HRP (green) and anti-Elav (red). Comparison of sc^10-1/Y (G,G') and sc^10-1/Y; C96-GAL4 UAS-P35/+ (I,I') indicates that neurons undergo apoptosis in sc^10-1 flies. Note the extra neurons in the PWM (compare with J). Also, unlike the PWM of a y w (wild-type) wing (J,J'), which is devoid of neurons, a C96-GAL4 UAS-P35/+ wing (K,K') is lined with cells that express neuronal markers.