Fig. 4. Phenotypic analysis of GS cells. (A) Characterization of cell surface antigens by flow cytometry. Red shading, specific antibody; unshaded, unstained control. (B) RT-PCR analysis of GS cells. GS cells on laminin were cultured for 9 days in the indicated conditions. (C) Suppression of Neurog3 transcript expression by GDNF/4OHT identified by real-time PCR analysis (mean±s.e.m.; n=3). Expression of Neurog3 was enhanced in both wild-type and myr-Akt-Mer GS cells in the absence of GDNF.