Fig. 6. Germline transmission of myr-Akt-Mer GS cells. (A) A recipient testis with EGFP fluorescence in the GS cell-derived germ cell colonies. Arrows indicate the green fluorescence of donor cell colonization. (B) Histological appearance of a recipient testis that underwent germ cell transplantation showing round spermatids. (C) Offspring derived from the microinjection of oocytes with round spermatids, showing fluorescence. (D) PCR analysis of tail DNA samples from the offspring. The Neo transgene is detected in two of the four offspring. Scale bars: in A, 500 µm; in B, 50 µm.