Fig. 2. Pygo2 is required for lens development in the mouse.
(A-C) Hematoxylin and Eosin-stained sections of eyes in wild-type (WT)
and Pygo2-/- mice show mildly (B, small lens) and severely
(C, no lens) affected mutants. (D-F) GFP fluorescence in whole-mount
embryos again showing small (E) or absent (F) lenses in the mutant.
(G-I) Eye region cryosections labeled for the lens marker
ß-crystallin (green) and for nuclei with Hoechst 33258 (blue). A
small group of ß-crystallin-positive cells is indicated by the arrow in
I. (J,L) Whole-mount embryos showing the WT and gross
Pygo2-/- phenotype. The arrow in L indicates pigmented
tissue within the eye cup. (K) Box plot of the lens:optic cup diameter
ratio in allelic series of Pygo1 and Pygo2 mutants. Each bar
represents measurements from ten eyes in five embryos. One-way ANOVA indicated
statistically significant changes as compared with the wild-type control
(**, P
0.01
0). ret, retina; mes, mesenchyme; pce,
presumptive conjunctival epithelium; pr, presumptive retina.