Fig. 3. Pygo2 regulates Pax6 expression in the mouse lens placode. (A-P) Eye region cryosections labeled for nuclei with Hoechst 33258 (A-N, blue), Pax6 (A-F, red), GFP (G,H, green), Sox2 (I,J, red), Pygo2 (K-N, red) or unlabeled differential interference contrast (DIC) images (O,P). The arrowhead in N (lower panel) indicates a small group of Pygo2-labeled cells in the presumptive lens. ple, presumptive lens ectoderm; ov, optic vesicle; lp, lens placode; pi, lens pit; pr, presumptive retina. A gray line between panels indicates that different channels of the same image are displayed. (Q) Box plot showing the ratio of lens to optic cup diameter in E12.5 embryos of the indicated genotypes. Mutant values (green and red bars) were significantly different (^**, P0.01) from the wild-type and Le-cre controls (yellow bars). In addition, the Le-cre conditional mutant (green bar) is significantly different (^**, P0.01) from the germline null (red bar). The wild-type control value incorporates the ten samples from Fig. 2K and a new set of 12 control values from this experiment which yielded the Le-cre conditional mutants. Germline null values are reproduced from Fig. 2K for the purposes of comparison.