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Figure 4


Fig. 4. Mesenchymal Pygo2 participates in lens development. (A,C,G,I) Whole-mount mouse embryos visualized for GFP (A,C) or in bright-field (G,I). (B,D-F,H,J-N) Unlabeled, DIC-illuminated cryosections (H,J), and cryosections labeled for nuclei with Hoechst 33258 (B,D-F, blue), Ap2{alpha} (B,D,K-N, red), GFP (B,D, green), Pygo2 (E,F, red) or Pax6 (K-N, green). A gray line between panels indicates that different channels of the same image are displayed. In D, the red channel within the dashed box has been enhanced to show weak Ap2{alpha} immunoreactivity in the ocular mesenchyme, the asterisks indicate GFP-negative blood vessels, and the arrow indicates remaining mesenchymal cells between presumptive lens and retinal epithelia. In E,F, the dashed line encloses approximately equivalent regions of the OM. In F, arrows point to Pygo2-positive blood vessels. In M,N are shown the green (Pax6) and red (Ap2{alpha}) channel intensities for a line interval passing through the nuclei of the lens placode. The bracket indicates the regions in the lens placode with a reduced Pax6:Ap2{alpha} ratio in the Pygo2 mutant. ple, presumptive lens ectoderm; ov, optic vesicle; om, ocular mesenchyme; lp, lens placode; pr, presumptive retina.