Fig. 6. ß-catenin/Wnt signaling in the OM is not required for lens development. (A-F) Whole-mount mouse embryo heads and eye region sections stained for ß-gal activity. The section shown in E defines the regions where quantification was performed for Fig. 7A. Dashed green line, dorsal neural tube (DNT); dashed purple line, optic vesicle (OV); dashed red line, ocular mesenchyme (OM). (G,H) Cryosections labeled for ß-catenin (red), Pax6 (green) and nuclei (blue). The dashed lines enclose regions of OM negative for ß-catenin immunoreactivity. Asterisks indicate blood vessels that remain immunoreactive to ß-catenin. (I-L) Whole-mount (I,K) and DIC-illuminated eye region sections (J,L). ov, optic vesicle; 1ba, first branchial arch; ple, presumptive lens ectoderm; om, ocular mesenchyme; lp, lens placode; pr, presumptive retina. A gray line between panels indicates that different channels of the same image are displayed.