Fig. 4. Interaction of atswc6 with other flowering-time mutants. Comparison of Arabidopsis flowering phenotype in (A) ld-1 and ld-1 atswc6,(B) fca-9 and fca-9 atswc6, (C) flc-3 and flc-3 atswc6, (D) ft-1 and ft-1 atswc6. Photographs were taken after 40 days in A and B, 20 days in C and 35 days in D. All plants were grown in long-day conditions. (E) Northern blot analysis of FLC and SOC1 in single and double mutants. The transcript level of FLC was reduced by approximately half in double mutants (fca-9 atswc6, ld-1 atswc6) as compared with each single mutant. (F) RT-PCR analysis of FLC and SOC1 in ft-1 and ft-1 atswc6. TUB was used as an internal control. In E and F, RNA was extracted from 10-day-old plants grown in long day conditions.