Fig. 4. SKN-1 asymmetry is disrupted in eel-1(-) and efl-1(-);eel-1(-) C. elegans embryos. (A,B) The posterior:anterior ratio (Post/Ant) of nuclear SKN-1 staining was calculated for individual embryos of the given genotype at the 2-cell (A) or 4-cell (B) stage. (C,D) Nuclear SKN-1 staining was quantitated in equivalent blastomeres for pairs of eel-1(RNAi) and wild-type embryos, and the eel-1(-) to wild-type ratio (eel-1/WT) was calculated for the anterior and posterior blastomere(s). Variability was minimized by performing individual comparisons between pairs of embryos from a single slide: 2-cell (C) and 4-cell (D) stage. The mean±s.e.m. is shown.