Fig. 4. Viable En1 and En2 double-mutant adult mice have midbrain and cerebellar defects. (A-D) Dorsal posterior views of adult brains of (A) wild-type, (B) En2^-/-, (C) En1^-/+;En2^-/+ and (D) En1^-/+;En2^-/- mutant mice. (E-L) Cresyl Violet-stained sagittal sections of the cerebella shown in A-D at the level of the vermis (E-H) and hemispheres (I-L), as indicated in A by the red and black dashed lines, respectively. Black and red arrowheads indicate the anterior and posterior foliation defects, respectively. Blue and red asterisks indicate hemisphere foliation defects and truncation in the IC, respectively. See Fig. 2 legend for midbrain and cerebellum annotation.