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Figure 2


Fig. 2. Colocalization and segregation of Numb and transitin in mitotic neuroepithelial cells. (A) Cortical colocalization of Numb and transitin. Pallium region of an embryonic day (E)5 chick telencephalon was stained with anti-Numb (green), 7B3 anti-transitin (purple) and DAPI nuclear dye (blue). Various phases of mitosis are shown. The apical ventricular surface is towards the bottom of each panel. Although Numb and transitin localize in the basal side of the prophase cell, both symmetric and asymmetric localization/segregation are observed in meta-, ana- and telophase. (B) Symmetric (above) and asymmetric (below) localization of Numb in relation to the spindle pole in anaphase neuroepithelium (NE) cells. Anti-{gamma}-tubulin staining (purple, arrowheads) is overlaid with anti-Numb (green) and DAPI (blue) staining. The predicted cleavage plane is indicated by a white line. (C,D) The proportion of asymmetrically localized Numb (C) and transitin (D) in mitotic NE cells of E5 telencephalic pallium (purple) and ventral metencephalon (green). Three embryos (approximately 300 mitotic NE cells/embryo) were examined to obtain each bar.