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Figure 6


Fig. 6. Dynamic lateral transport of Trans(1-327)-d1EGFP in mitotic neuroepithelial cells. Blue is DAPI nuclear staining. Basal is up. (A,B) Trans(1-327)-d1EGFP (green) basally colocalizes with endogenous transitin (A) and Numb (B) in mitotic neuroepithelium (NE) cells (outlined). (C) Time-lapse analysis of sliced, cultured neuroepithelium co-transfected with Trans(1-327)-d1EGFP (green) and DsRed2-nuc (purple), indicating chromosomes. Cell shape is outlined. The border of Trans(1-327)-d1EGFP localization is indicated by arrowheads. A broken straight line in panel 8 indicates the cleavage plane. `Time=0' indicates the start point of observation.