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Figure 1


Fig. 1. Nkx6.1 is expressed in similar domains to Ngn3 and Pdx1 during pancreas development. (A-I) Immunofluorescence for the indicated proteins in the developing mouse pancreas at E10.5 (dorsal bud; A,D,G), E12.5 (B,H), E14.5 (C,E,I) and E18.5 (F). (A) Ngn3 (red) is co-expressed with Pdx1 (green) at E10.5 (arrows). (B) At E12.5, a subset of Ngn3+ cells does not express Pdx1 (arrowhead), but some Ngn3+ cells maintain Pdx1 expression (arrow). (C) At E14.5, the majority of Ngn3+ cells express low levels of Pdx1. However, some Ngn3+ cells are Pdx1-negative (arrowhead) and some express high levels of Pdx1 (arrow). (D-F) Nkx6.1 (red) is co-expressed with Pdx1 (green) at E10.5 (D), E14.5 (E) and E18.5 (F). Notably, Nkx6.1 is absent from Pdx1+ acini at E14.5 (E, arrow). (G-I) Ngn3 (green) is co-expressed with Nkx6.1 (red) at E10.5 (G, arrows), E12.5 (H) and E14.5 (I). Scale bar: 50 µm.