Fig. 5. Ftm is necessary for the establishment of a proper Gli3-190/Gli3-83 ratio in mice. (A) Immunoblots show the amounts of Gli3-190 and Gli3-83 in E11.5 embryos. In wild-type and Ftm^+/- embryos, the majority of Gli3 protein was processed to form Gli3-83, and only small amounts of Gli3-190 are detectable. In Ftm^-/- embryos, the amount of Gli-83 is equal to wild-type levels, but the amount of Gli3-190 is increased. Note that there is an increase in total Gli3 protein in Ftm^-/- embryos. The lower part shows the loading control with anti-tubulin antibody. (B) Graphical evaluation of the Gli3-190/Gli3-83 ratio in wild-type, Ftm^+/- and Ftm^-/- embryos. At least three embryos of each genotype have been analysed. (C) RT-PCR analysis of RNA from E11.5 embryos. The amount of Gli3 transcripts is increased in Ftm^-/- embryos in comparison with the wild type. The lower part shows the Hprt normalization. (D) Comparison of relative Gli3 transcription levels in wild-type and Ftm^-/- embryos (n=3). In Ftm^-/- embryos, the number of Gli3 transcripts is increased nearly twofold.