Fig. 1. Expression of Pfkp and Ldha mRNA and glycolysis in Gdf9^+/- Bmp15^-/- (double mutant, DM) and Bmp15^-/- cumulus cells. (A) Localization of Pfkp and Ldha mRNA was detected by in situ hybridization using 22-day-old eCG-primed, either WT or DM, mice. CC, cumulus cells; MG, mural granulosa cells; O, oocytes; TC, theca cells. (Top) Brightfield images. (Bottom) Darkfield images. Scale bar: 500 µm. (B) Relative mRNA levels of Pfkp and Ldha in cumulus cells of WT, DM, Bmp15^-/- or Gdf9^+/- mice were examined using real-time PCR. (C) Relative glycolytic activity in COCs of WT, DM, Bmp15^-/- or Gdf9^+/- mice was measured as their ability to metabolize [5-³H]-glucose to ³H₂O. Mean±s.e.m. The values indicated by different letters (a and b) are significantly different (P<0.05).