Fig. 7. Requirement for FGF signals to promote the expression of Pfkp and Ldha mRNA in OOX cumulus cells. (A) Dose-response of FGF8. OOX cumulus cells were cultured with (black circles) or without (white circles) oocytes (0.1 oocytes/µl) and treated with recombinant FGF8B, and expression levels of Pfkp and Ldha mRNA in the OOX cumulus cells were examined. As a control group, Pfkp and Ldha mRNA expression in OOX cumulus cells co-cultured with oocytes (2 oocytes/µl) is shown (black bars). (B) Effect of SU5402. OOX cumulus cells were co-cultured with oocytes (0.5 oocytes/µl) in the presence of either DMSO or SU5402 for 24 hours, and relative mRNA levels of Pfkp and Ldha in OOX cumulus cells were examined. (C) Reversibility of the effect of SU5404. OOX cumulus cells were co-cultured with oocytes (0.5 oocytes/µl) in the presence of either DMSO or SU5402. After 24 hours of culture, the OOX cumulus cells were washed thoroughly and cultured in a fresh medium for an additional 20 hours with freshly isolated oocytes (0.5 oocytes/µl), and relative mRNA levels of Pfkp and Ldha in the OOX cumulus cells were examined. Mean±s.e.m. The values indicated by different letters (a, b, c and d) are significantly different (P<0.05).