Fig. 2. Distribution of CYLD in Drosophila embryos and adults. (A-D) Detection of CYLD in embryos by immunohistochemistry. (A) At stage 7, CYLD is expressed in the anterior midgut rudiment (amg), in the posterior midgut rudiment (pmg) and in the mesoderm (ms). (B) At stage 12, CYLD is localized in the developing brain area (br), in the forming midgut (amg and pmg) and in the ventral nerve cord (vnc). (C) At stage 14, CYLD is detected at the brain (br), the ventral nerve cord (vnc), the midgut (mg) and the hindgut (hg). (D) Stage 12 embryos homozygous for the Df(2L)J21 deficiency, which deletes the CYLD locus, did not stain with the anti-CYLD antibody. (E-J) Detection of CYLD in adult tissues by immunofluorescence. (E) Negative control using a non-specific rabbit polyclonal antibody (anti-GFP) as primary antibody and the same secondary antibody used in F-J. Using the same settings as in E, endogenous CYLD levels were detected with a rabbit polyclonal anti-CYLD antibody on adult w1118 sagittal paraffin sections (8 µm). CYLD was detected in ovaries (F), in testes (G), in the fat body (H), in the antenna (I) and in the eye (J). amg, anterior midgut rudiment; br, brain; hg, hindgut; mg, midgut; ms, mesoderm; pmg, posterior midgut rudiment; vnc, ventral nerve cord.