Fig. 6. CS-GAGs are essential components for neurogenic precursor cells in vivo. (A,B) Photomicrographs of immunohistochemical stainings of E14.5 mouse forebrain cryosections 1 day after ventricular injection with ACSF control (upper panels) or ChABC (lower panels). The cortical precursor pool was examined for coexpression of BLBP and nestin (A) and for GLAST expression (B). Following ChABC treatment, immunoreactivities for nestin and BLBP significantly decreased, whereas more cells were GLAST-positive, in comparison with the situation following ACSF injection. Cell nuclei were counterstained with bisbenzimide and are shown in blue (DAPI). (C,D) Single-cell suspensions obtained from embryos injected with ACSF (dark gray) or ChABC (light gray) were plated and immunopositive cells determined after 2 hours. (C) ChABC treatment caused a 2-fold reduction in the number of nestin- and BLBP-positive cells, whereas GLAST-positive cells increased. (D). The relative fractions of cortical (Cor) and striatal (GE) Nsphs grown from single-cell suspension were reduced following ChABC (light gray) injection. Numbers are plotted with reference to the control-injected telencephali, taken as 100% (dark gray). ^*, P<0.05; ^**, P<0.01. Scale bar: 100 µm.