Fig. 6. Stat6 signalling is intact in KIM-2 cells. KIM-2 cells respond to
IL-4 (A) and IL-13 (B) in a dose-dependent manner, confirmed by
immunoblotting using phosphorylated (p)Stat6 and Stat6 antibodies. (C)
KIM-2 medium was changed and then cells were harvested at various time points
after treatment with or without 40 ng/ml of recombinant murine IL-13
(rmIL-13). Quantitative real-time-PCR was performed for
Il4r
1 and Gata3; * denotes
Student's t-test P value of <0.02. (D) Immunoblots
for the same time course were performed for pStat6, Stat6 and -tubulin.
(E-G) KIM-2 cells were treated with brefeldin A (10 µg/ml) for 4
hours, fixed and stained with anti-IL-4 (E) or anti-IL-13 (F) antibodies or no
antibody (G).