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Figure 4


Fig. 4. p53 family members do not contribute to the Notch1-deficient follicular phenotype. (A) Quantitative reverse transcriptase (qRT)-PCR of p63 and p73. (B) Immunohistochemistry of p73 in hair follicles of anagen-deleted Notch1 (anN1), embryo-deleted Notch1 (em-N1) and wild-type (WT) littermates. (C) qRT-PCR with Notch1, Trp53 (p53) and p53 target genes [Cdkn1a (p21), Igfbp3 and Scotin] using total RNA from the hair bulbs of the genotypes specified (emN1;p53-/-indicates Msx2Cre;Notch1flox/flox;Trp53-/-). Note the sustained increase in Scotin and Igfbp3 expression in the absence of p53. Error bars represent s.e.m. Scale bar: 50 µm.