Fig. 3. CMFDA tracer injections into the subpallium and pallial-subpallial boundary (PSB) label cells in the pallium. (A-D'') Four coronal slices from stage 15 turtle embryos were labeled from the VZ of the PSB (A), LGE (B) or MGE (C, rostral and D, caudal) with CMFDA-coated bamboo fibers (black or white stars), cultured for 5 days, fixed in PAF and immunoreacted with Darpp-32 antibodies (A',B',C',D'). (A'',B'',C'',D'') Merged images (CMDFA, green; Darpp-32, red). (A) A PSB injection ventral to the DVR and dorsal to the Darpp-32-positive LGE area (A') labeled cells in the DVR and LC but not in the DC. (B) Tracer placement into the LGE labeled numerous cells oriented radially in the Darpp-32-positive area (B') and few cells in the pallium. (C,D) MGE injections ventral to the Darpp-32-positive area (C',D') labeled cells between the Darpp-32-positive LGE and DVR, in the LC and along the VZ of the DVR (C'',D''). A few labeled cells are present in the DC. Scale bar: 200 µm. (E-H') Four coronal slices from turtle embryos at stage 17 (E-G') or 19 (H,H') were labeled with CMDFA-coated bamboo fibers (black or white stars) placed into the VZ of the PSB (E,H), LGE (F) or MGE (G) and stained for Darpp-32 (E'-G') or calbindin (H') immunoreactivity after fixation. PSB placements labeled a very large number of cells in the PSB (E,H), and labeled scattered cells in the DVR and cortex (LC, DC). LGE placements at stage 17 (F) labeled numerous cells in the pallium, at the PSB and along the pallial VZ. By contrast, MGE placements (G) no longer labeled cells in the pallium, and labeled few cells along the PSB. Scale bars: 200 µm in E-G'; 500 µm in H,H'.