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Figure 1


Fig. 1. Growth phenotype of wild-type and er-family mutant Arabidopsis. (A-H) Wild-type and er-family mutant inflorescence stems. (A) Wild type (WT); (B) er-105; (C) er-105 erl1-2; (D) er-105 erl1-2 erl2-1; (E) er-105 erl1-2 erl2-1/+; (F) er-105 erl1-2/+ erl2-1; (G) er-105 erl1-4 erl2-1/+; and (H) er-105 erl1-1 erl2-1/+. Wild type and all combination mutants (A-C,E-F) except er-105 erl1-2 erl2-1 triple mutant (D) maintain proper floral organ patterning and elongation. Among the combinations, er-105 erl1-2 erl2-1/+ (E), er-105 erl1-4 erl2-1/+ (H) and the triple mutant (D), are female sterile. Scale bar: 1 cm. (I) Schematic of the ERL1 gene with location of T-DNA insertions for erl1-1 and erl2-1, and the point mutation for erl1-4. Exons are indicated as vertical bars, introns as lines. The T-DNA right border (RB) is boxed. (J) RT-PCR analysis of four separate erl1-1 plants with reduced levels of ERL1 transcript compared with wild type. ACTIN 2 serves as a positive control.