Fig. 8. The Pros6T¹ mutant delays the disappearance of histone H2AvD but protamine incorporation is not affected. Images are of increasing age, left to right. Wild-type (A-F,M-R) or Pros6T¹ (G-L,S-X) testes expressing either H2AvD-GFP (D-F,J-L; green) or ProtA-GFP (P-R,V-X; green) were stained with TO-PRO3 (A-C,G-I,M-O,S-U; red) to visualize nuclei. In wild-type testes, H2AvD-GFP signal is barely detectable in elongating and condensing nuclei (D), and completely disappears in mature nuclear bundles (E) and nuclei in individualized sperm (F). However, elongating and condensing nuclei in Pros6T¹ testes still contain significant amounts of H2AvD-GFP (J), although the GFP signal disappears in mature nuclear bundles (K) and curled nuclei (L). ProtA-GFP is highly expressed in elongating and condensing nuclei in wild-type testes (P), and persists in the mature nuclear bundles (Q) and nuclei in individualized sperm (R). In Pros6T¹ testes, ProA-GFP is observed in elongating and condensing nuclei (V), mature nuclear bundles (W) and curled nuclei (X). Scale bar: 20 µm.