Fig. 6. Hair follicle in the absence of Gata3. (A,B) In situ mRNA hybridization of Krtap16-7 at P11; insets show normal cortex expression in the wt (A), whereas expression spreads into the medulla in K14-Gata3^-/- hairs (B). (C,D) Cortical cells recognized by AE13 antibody were no longer separate from the pigmented cells in K14-Gata3^-/- HFs and those HFs exhibited expanded precortex and cortex as compared with wt HFs. Arrowheads indicate lack of separation of cortical cells from the pigmented cells. DAPI was used to mark the nuclei. (E,F) AE15 antibody recognizes trychohyalin IRS and medulla of control HFs (E) and only wisps of cells in K14-Gata3^-/- IRS (F). There is a lack of separation of AE15-positive cells from K14-positive cells in K14-Gata3^-/- HFs as compared with control (E,F, insets). (G,H) Expression of Crym at P11 in control (G) and K14-Gata3^-/- skin (H); arrows in G point to expression in IRS. Scale bar in G: 200 µm for A,B,G,H; 100 µm for C,D; 50 µm for E,F.