Fig. 1. APC/C function is required for Miranda localisation to the basal cortex. (A-D,H,I) In ida^PL17 mutant neuroblasts (NBs), Miranda is exclusively cytoplasmic during prophase (B) and accumulates pericentrosomally during metaphase (D) and anaphase (I), rather than forming a cortical crescent as in wild-type larval NBs (A,C,H). (E) Expression of a GFP::Ida fusion protein fully rescues the Miranda localisation defects observed in ida^PL17 mutant NBs. (F,G) Loss-of-function mutants for APC/C subunits cdc27 (F) and morula (mr, APC2; G) also show Miranda pericentrosomal accumulation. (J-M) Miranda mislocalisation occurs independently of both centrosome function and microtubules. cnn; ida double mutants still accumulate pericentrosomal Miranda (L) and Miranda mislocalisation in ida mutant NBs is insensitive to colchicine treatment, which depolymerises microtubules (M). Broken circle in M indicates the outline of the NB. Miranda, red (A-M); PH3, green (A,B); GFP, green (E); Cnn, green (J-M); DNA, blue (A-M). (N) Quantification of Miranda pericentrosomal mislocalisation in allelic combinations of ida mutants. Scale bar: 10 µm.