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Figure 2


Fig. 2. The dorsal midline fails to develop in Bmpr1a;Bmpr1b double-mutant mice. Expression analysis using radioactive RNA in situ hybridization on E11.5 (A-F) and E10.5 (G-J) coronal sections. (A-F) The mutant lacks expression (arrowheads) of markers for: the choroid plexus, Ttr (A,B); the cortical hem, Wnt2b (C,D); and for both choroid plexus and hem, Msx1 (E,F). (G-J) The expression of Bmp4 in the dorsal midline and adjacent neuroepithelium is maintained in the mutant (G,H), although the domain of expression is reduced. Note the rostroventral midline expression of Bmp4 (arrow in G). The ventral and dorsal midline expression of Zic2 is maintained in the mutant (arrowheads in I,J). (K,L) TUNEL staining for apoptotic cells is absent at the dorsal (arrowheads) and ventral (arrows) midline in the E10.5 Bmpr1a;Bmpr1b mutant. Scale bars: 150 µm.