Fig. 6. Slit topography correlates to aCC dendrogenesis in robo/robo mutant. (A) Detergent-free immunocytochemistry with anti-HRP (green) and anti-Slit (purple) in robo⁴/robo⁴ embryos at hour 14. (B) Slit concentration topography (neuropilar portion, normalized to the midline level in +/+ at hour 14; see Fig. 3F) in wild type (+/+) (n=12) and robo⁴/robo⁴ (n=12) embryos at hour 14. Arrows indicate the center of the neuropilar concentration. (C) Scattergraphs (up to 10 aCCs per genotype) of dendritic tips in +/+ (n=14) and robo⁴/robo⁴ (n=14) at hour 17. Each horizontal line indicates an individual aCC neurons and circles indicate the positions of dendritic tips along its axons. Scale bar: 5 µm.