Fig. 9. Virtually wild-type neuropil but reduced Slit leads to small aCC dendrites in slit/+ and Sdc/Sdc mutants. (A,B) Detergent-free immunocytochemistry with anti-HRP (green) and anti-Slit (purple) in slit²/+ (A) and Sdc¹⁰⁶⁰⁸/Sdc¹⁰⁶⁰⁸ (B) embryos at hour 14. (C,D) Tracing s of slit²/+ (C) and Sdc¹⁰⁶⁰⁸/Sdc¹⁰⁶⁰⁸ (D) aCCs at hour 17. (E) Slit concentration topography in +/+ (n=12), slit²/+ (n=5), Sdc¹⁰⁶⁰⁸/Sdc¹⁰⁶⁰⁸ (n=12) and slit²/slit² (n=3) at hour 14. Arrows and horizontal lines indicate the maximum numbers of dendritic tips per bin. (F) Histogram of the number of dendritic tips in +/+ (n=14), slit²/+ (n=12), Sdc¹⁰⁶⁰⁸/Sdc¹⁰⁶⁰⁸ (n=14) and slit²/slit² (n=9) aCCs at hour 17. Arrows and horizontal lines indicate the neuropilar peaks. Scale bar: 5 µm.