Fig. 4. Abnormal testicular development of the PU.1-negative mouse
mutant. (Aa-g,Ba-g) Testis sections of
PU.1+/+ (a,c,e) and PU.1G/G (b,d,f)
littermates at E15.5 (A) and E17.5 (B). (Ag,Bg) The average number of
gonocytes per transverse tubule section, for both developmental times.
(C) RT-PCR assay for vasa and stella expression performed on RNA
prepared from E18.5 PU.1G/G, PU.1G/+ and
PU.1+/+ fetal testis. Scale bars: 50 µm.