Fig. 4. Effect on global autofluorescence of inhibition of mitochondrial respiration and pyruvate uptake. (A) Timecourse of changes in global NAD(P)H and FAD⁺⁺ autofluorescence in a mature MII oocyte incubated in H-KSOM/AA alone (n=18 oocytes). Pyruvate is added after inhibition of mitochondrial respiration with rotenone and cyanide (CN^-, as indicated). (B) Timecourse of changes in global NAD(P)H and FAD⁺⁺ autofluorescence in a mature MII oocyte after cinnamate (CIN) perfusion (n=15 oocytes). The efficacy of CIN as an inhibitor of mitochondrial pyruvate uptake is confirmed by the lack of effect of the subsequent addition of pyruvate on FAD⁺⁺ autofluorescence. (C) Effect of CIN perfusion on global autofluorescence of a mature MII oocyte after inhibition of LDH with oxamate (n=12 oocytes). Rotenone was added to a final concentration of 15 µmol/l. The x-axes indicate time in minutes.