Fig. 5. Pax6 overexpression affects the formation of superficial cortical layers. (A,B) Coronal sections through the cortex of (A) a wild-type and (B) a PAX77 mouse at P7, stained with cresyl violet. Borders between adjacent cortical layers were identified based on differences in cytoarchitecture. (C-E) Thickness of cortical layers I, II-IV (combined), V and VI in the (C) rostral, (D) central and (E) caudal cortex of wild-type and PAX77 mice at P7. The thickness of layers II-IV is significantly decreased in the rostral and central cortex of PAX77 mice compared with wild type. Scale bar: 300 µm.