Fig. 9. Production of secretory proteins in Fgfr2^cn prostates remained androgen dependent. (A) Profiles of secretory proteins. The PBS-extracted proteins from the dlp of 2-month-old mice were separated on 5-20% gradient SDS-PAGE and stained with Coomassie Brilliant Blue G250. (B) Expression of probasin and PSP94 in Fgfr2^cn prostates. The PBS-extracted proteins from castrated or uncastrated mice were separated on SDS-PAGE and were western blotted with anti-probasin and anti-PSP94 antibodies, as indicated. (C) Total RNA was extracted from adult Fgfr2^cn and control prostates, and expression of probasin and PSP94 was determined by real-time RT-PCR. Expression levels were normalized to ß-actin loading controls. The expression of each gene in control prostates was set to 1. Data are mean±s.d. of three independent experiments. F/F, homozygous Fgfr2^flox mice; CN, Fgfr2^cn mice.