Fig. 2 . Non-synaptic nuclei are disorganized in Syne-1^-/- mice. (A-C) A single muscle fiber was teased from the tibialis anterior and simultaneously stained with DAPI (blue) and anti-SUN2 (green). Myonuclei were found to be distributed evenly in Syne-1^+/- (A), but formed clusters (B) and arrays (C) in Syne-1^-/- mice. (D) Statistical data shows that more than 99% of fibers in Syne-1^-/- mice formed three or more nuclear clusters outside NMJs (n>110 for each group). Scale bar: 25 µm.