Fig. 7 . Phrenic nerves display longer branches in Syne-1^-/- and Syne DKO mutants. (A-D) Whole-mount diaphragms of E18.5 embryos were stained with anti-neurofilament and anti-synaptophysin (green). The right hemi-diaphragm of each genotype is shown. Longer branches are obvious in Syne-1^-/-; Syne-2^+/- and Syne-1^-/-; Syne-2^-/- samples. (A'-D') Enlarged views of asterisk-indicated regions in A-D, showing the elongated phrenic nerve branches (green) and the broader endplate bands (red) in Syne-1^-/-; Syne-2^+/- and Syne-1^-/-; Syne-2^-/- diaphragms (C',D'). Scale bars: 500 µm in D for A-D; 50 µm in D' for A'-D'.